Mouse Esophageal Cancer Organoid Construction Kit CAT : MOCK028

Category：Tumor organoids Kit
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Product Overview
Instructions

“One-stop” Organoid Construction Kit

Product Description

The “One-stop” Organoid Construction Kit developed by Jiyan Biotech is an all-in-one solution for organoid construction, integrating tissue sampling, organoid culturing, subculturing, cryopreservation, and resuscitation. It is characterized by its user-friendly nature and ready-to-use design, achieving a remarkably high success rate of over 90% in organoid construction. There are over 50 varieties of human/mouse/monkey organoid construction kits available to meet diverse requirements. Additionally, on-site technical support is provided, and we offer a free reconstruction service in case the organoid construction fails.

This product equips customers with all the necessary consumables and reagents throughout the entire experimental process, minimizing the time consumption on non-experimental procedures. According to the operation manual of this product, provided that the sampling prerequisites are fulfilled and the uncontrollable factors such as sample-induced contamination are eliminated, the success rate of organoid construction can exceed 90%.

Mouse Esophageal Cancer Organoid Construction Kit

Kit Specifications

Table 1.1 Reagents Contained in Kit

Cat. #	Name	Size	Qty.	Stg. Cond.
B001-25	Tissue Storage Solution	25mL	1	2-8℃
B002-250	Tissue Rinsing Solution	250mL	1	2-8℃
B003-5	Tissue Fixation Solution	5mL	1	2-8℃
D001-1-50	Digestion Solution I	50mL	1	-20℃
B004-250	Organoid Washing Solution	250mL	1	2-8℃
B005-5	Red Blood Cell Lysis Solution	5mL	1	2-8℃
B006-5	Rinsing Solution	5mL	2	2-8℃
B007-01	Trypan Blue	100μL	1	2-8℃
M001-1	Matrigel	1mL	2	-20℃， avoid repeated freez-thaw, aliquot as needed upon first use.
MOCM028-100	Mouse Esophageal Cancer Organoid Culture Medium	100mL	1	Store at -20℃ for 6 months, 2-8℃ for 2 weeks, avoid repeated freez-thaw, aliquot as needed upon first use.
S001-1	Supplement I	1mL，100×	1	-20℃， avoid repeated freez-thaw, aliquot as needed upon first use.
B008-25	Organoid Recovery Solution	25mL	1	2-8℃
D002-10	Digestion Solution II	10mL	1	2-8℃
B009-25	Organoid Cryopreservation Solution	25mL	1	2-8℃

Table 1.2 Consumables Included in Kit

Name	Size	Qty.
Sterile Ophthalmic Scissors	10cm Curved Scissors	1
Sterile Ophthalmic Forceps	10cm Ophthalmic Straight Forceps	1
Sterile Centrifuge Tube	1.5mL	10
Sterile Pasteur Pipette	3mL Extended	15
Sterile Cell Strainer	100μm	2
Suspension Cell Culture Plate	24 holes, individually wrapped	2
Sterile Cryovial	2mL	10

Storage Conditions

The Mouse Esophageal Cancer Organoid Culture Medium should be stored below -20°C and has a shelf life of 6 months. It can be stored at 2-8°C for two weeks.

The rest of the reagents should be stored according to their specific conditions.