Research on the Modeling of Fatty Liver Using Organoids

I. Introduction of the model

Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) is a chronic metabolic stress liver disease closely related to insulin resistance and genetic susceptibility, with a disease spectrum that includes non-alcoholic hepatic steatosis, non-alcoholic steatohepatitis and its associated cirrhosis and hepatocellular carcinoma. MAFLD is not only an important cause of disability and death from liver disease , it is also closely associated with a high prevalence of metabolic syndrome, type 2 diabetes mellitus, atherosclerotic cardiovascular, cerebral, renal and vascular diseases and colorectal tumors. Overnutrition is the main pathogenesis of MAFLD, and this model can simulate MAFLD in vitro, providing a reliable experimental basis for studying the pathogenesis of fatty liver and drug screening.

II. Identification of lipid droplets in organoid MAFLD

·  Nile Red appraise

Figure A Nile Red staining of liver organs after 5 days of FFAs bar=50μm

·  Oil Red O Identification

Figure B: FFAs treated for 1 day, simulating early fatty liver

Figure C: FFAs treatment for 5 days, simulating moderate/severe fatty liver

Figure D: FFAs treatment for 5 days,oil red O staining results

V:control group; T: FFAs treated group.

· Identification of triglycerides

· Comparative testing of FFAs

III. Conventional evaluation indicators