Research on Enteritis Modeling of Organoids

I. Introduction to the model

Inflammatory Bowel Disease (IBD) is a non-specific chronic inflammatory disease of the colon or gastrointestinal tract, including ulcerative colitis (UC) and Crohn's disease (CD), with similar clinical symptoms including recurrent diarrhea, abdominal pain, intestinal bleeding/bloating, fever, and weight loss. The pathogenesis of IBD is unclear, and studies have shown that a variety of factors, including susceptibility genes, the immune system, the external environment, and intestinal microorganisms, are associated with the development of the disease. Dextran sodium sulfate (DSS)-induced enterocolitis model is the most widely used chemically induced IBD model.DSS induces inflammation in the model, leading to intestinal epithelial cell damage, altered cell permeability, disruption of cell membrane integrity, and increased apoptosis. Cytokines are released from non-specific immune cells, which ultimately leads to the disruption of the integrity of the mucosal barrier.

II. A case of identification of inflammatory indicators in organoid enteritis models

Results:

DSS 1% induced an inflammatory response in organoids, which subsequently decreased after reaching the peak point of the inflammatory response.

III. Conventional evaluation indicators

IV. Other inflammation modeling services