CSwell 600

Category：Organ-on-a-Chip
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Product Overview
Instructions

CSwell 600

Culture Dishes for Rapid and Simple Preparation of 3D Cell Spheres (Embryoid Bodies, Organoids)

Product Description

In vitro construction of 3D tissues and cells of specific types is crucial in life sciences and biomedicine. CSwell 600, a specialized miniature culture chamber, is designed for preparing 3D cell spheres and enables the in vitro construction of stem cell-derived embryoid bodies and various organoids. It features a standard array of micropores, facilitating high-throughput production of uniformly sized 3D cell spheres. The size of the cell spheres can be flexibly controlled by adjusting the cell concentration to meet different organoid construction needs.

Note: This product is suitable for 3D cultivation of most stem cells and tumor cells. For highly adhesive cells, it should be used in conjunction with a low-adhesion surface modification solution.

Cat. #	Name	Size
KIT000-0001	CSwell 600	4 wells
KIT000-0001	Low-Adhesion Surface Modification Solution	3 mL

Note: Do not expose this product to organic solvents, except ethanol. This product has been sterilized before leaving the factory, and autoclaving is prohibited.

Storage

It can be stored at room temperature (15 - 25°C) and has a shelf life of 12 months.

The Cell Quantity Required for the Preparation of 3D Cell Spheres

The cell seeding quantity required for each CSwell 600 = the number of cells expected to form cell spheres × 580

Table 1. Cell Seeding Quantity Requirement Table for CSwell 600

Expected No. of cells in each micropore	Corresponding cell seeding suspension concentration (cells/ml)
50	2.9×10⁴
100	5.8×10⁴
200	1.16×10⁵
500	2.9×10⁵
1000	5.8×10⁵
2000	1.16×10⁶
5000	2.9×10⁶
10000	5.8×10⁶

This product recommends that the number of cells per micropore be within the range of 100 - 10,000 cells.

The volume of the inoculated cell suspension for each CSwell 600 culture chamber is 1 ml.

Note: When preparing the cell suspension, try to avoid the multi-step dilution method to ensure the accuracy of the cell concentration.

Precautions

This product is applicable to the 3D cultivation of most types of stem cells, tumor cells, etc. However, for cells with strong adhesiveness, it needs to be used in combination with Anti-Adherence Rinsing Solution.

This product is only for scientific research purposes and must not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals. It should not be stored in ordinary residences.

For the safety and health of laboratory personnel, it is recommended to wear laboratory coats, masks and disposable gloves during the experiment.