“One-stop” Organoid Construction Mini Kit
Product Description
The “One-stop” Organoid Construction Kit developed by Jiyan Biotech is an all-in-one solution for organoid construction, integrating tissue sampling, organoid culturing, subculturing, cryopreservation, and resuscitation. It is characterized by its user-friendly nature and ready-to-use design, achieving a remarkably high success rate of over 90% in organoid construction. There are over 50 varieties of human/mouse/monkey organoid construction kits available to meet diverse requirements. Additionally, on-site technical support is provided, and we offer a free reconstruction service in case the organoid construction fails.
This product equips customers with all the necessary consumables and reagents throughout the entire experimental process, minimizing the time consumption on non-experimental procedures. According to the operation manual of this product, provided that the sampling prerequisites are fulfilled and the uncontrollable factors such as sample-induced contamination are eliminated, the success rate of organoid construction can exceed 90%.
Mouse Ovarian Cancer Organoid Construction Mini Kit
Mini Kit Specifications
Table 1.1 Reagents Contained in the Mini Kit
Cat. # | Name | Size | Qty. | Stg. Cond. |
B001-5 | Tissue Storage Solution | 5mL | 1 | 2-8℃ |
B001-10 | Tissue Storage Solution | 10mL | 1 | 2-8℃ |
B002-100 | Tissue Rinsing Solution | 100mL | 1 | 2-8℃ |
B003-1 | Tissue Fixation Solution | 1mL | 1 | 2-8℃ |
D001-1-10 | Digestion Solution I | 10mL | 2 | -20℃ |
B004-60 | Organoid Washing Solution | 60mL | 1 | 2-8℃ |
B005-1 | Red Blood Cell Lysis Solution | 1mL | 1 | 2-8℃ |
B006-5 | Rinsing Solution | 5mL | 1 | 2-8℃ |
B007-01 | Trypan Blue | 100μL | 1 | 2-8℃ |
M001-05 | Matrigel | 500μL | 1 | -20℃,avoid repeated freez-thaw, aliquot as needed upon first use. |
| MOCM024-30 | Mouse Ovarian Cancer Organoid Culture Medium | 30mL | 1 | Store at -20℃ for 6 months,2-8℃ for 2 weeks, avoid repeated freez-thaw, aliquot as needed upon first use. |
S001-02 | Supplement I | 200μL,100× | 1 | -20℃,avoid repeated freez-thaw, aliquot as needed upon first use. |
B008-5 | Organoid Recovery Solution | 5mL | 1 | 2-8℃ |
D002-1 | Digestion Solution II | 1mL | 1 | 2-8℃ |
Table 1.2 Consumables Included in the Mini Kit
Name | Size | Qty. |
Sterile Ophthalmic Scissors | 10cm Curved Scissors | 1 |
Sterile Ophthalmic Forceps | 10cm Ophthalmic Straight Forceps | 1 |
Sterile Centrifuge Tube | 1.5mL | 10 |
Sterile Pasteur Pipette | 3mL Extended | 15 |
Sterile Cell Strainer | 100μm | 2 |
Suspension Cell Culture Plate | 24 holes, individually wrapped | 2 |
Storage Conditions
The Mouse Ovarian Cancer Organoid Culture Medium should be stored below -20°C and has a shelf life of 6 months. It can be stored at 2-8°C for two weeks.
The rest of the reagents should be stored according to their specific conditions.