B007-Trypan Blue

Trypan Blue

Product Description

Storage

Store at 2-8℃ for 12 months.

Instructions for Use

(1) Cell Collection:

For adherent cells, first digest the cells with trypsin and/or EDTA. For suspension cells, the cells can be collected directly. Centrifuge the collected cells at 1000 - 2000 g for 1 minute, discard the supernatant, and then resuspend the cells in 1 mL or an appropriate volume of cell resuspension solution according to the amount of cells.

(2) Trypan Blue Staining:

Pipette 100 μL of the resuspended cells into a plastic centrifuge tube, add 100 μL of Trypan blue staining solution (2X), gently mix them evenly, and stain for 3 minutes (a staining time of 3 minutes is sufficient, although the staining time can be extended a bit longer, but it should not exceed 10 minutes).

Note: For cells resuspended in cell culture medium, an equal volume of Trypan blue staining solution (2X) can also be directly added for staining.

(3) Counting:

Pipette a small amount of the stained cells and count them using a hemocytometer. Generally, if a relatively precise quantification is required, at least 500 cells should be counted for each cell sample, and both the number of blue cells and the total number of cells should be recorded.

Cell viability = (Total number of cells - Number of blue cells) / Total number of cells × 100%.

Precautions

This product is strictly reserved for scientific research and shall not be applied to clinical diagnosis, treatment, food, or pharmaceuticals. Additionally, it is prohibited from being stored in residential premises.