New Product 丨Specialized Tissue Embedding Gel for Organoids (SE-Gel)

New Product Release of Jiyan Biotech

Specialized Tissue Embedding Gel for Organoids (SE-Gel)

Jiyan Biotech focuses on the field of precision oncology medical services, committed to the construction of tumor organoid models and the development of related chips. In scientific research, after the successful construction of organoids, it is necessary to conduct tissue morphology and specific marker identification. During this process, the technical challenges in embedding lie in embedding and sectioning. Traditional embedding methods that require high - temperature heating involve complex melting and solidification processes, with difficult - to - master time control, demanding constant attention. When using paraffin embedding, due to the small size of organoids, they are extremely likely to be lost, making it difficult to obtain satisfactory tissue sections, and it is hard to locate the target tissue after sectioning.

To address the above pain points, Jiyan Biotech has launched SE - Gel, a specialized tissue embedding gel for organoids. It enables the visualization and traceability of organoid embedding, minimizes the loss of the target tissue during sectioning, features simple operation and short time - consumption. SE - Gel boosts the cutting - edge technologies in biomedicine and fills the market gap.

Organoids Tissue Embedding Glue (SE-Gel) S-001

For more information, please visit: https://en.jiyanbio.com/product/840.html

Jiyan Biotech has independently developed a set of easy - to - operate specialized embedding gel for organoids using extracellular matrix derived from porcine tissues. It can embed a small amount of organoids in several tens of microliters or even a dozen microliters of embedding gel. Through pre - staining, it ensures that the positions of organoids and gel droplets can be observed during sectioning, preventing the loss of important samples during trimming.

The embedding process can be completed in a conventional laboratory without the need for special instruments, reagents, or consumables. Multiple samples can be embedded within 1 hour. The operation process and related reagents cause no damage to the organoids. The embedded organoids can be regarded as tissue blocks and directly undergo subsequent OCT - embedding frozen sectioning according to the normal tissue embedding procedure, or wax blocks can be prepared for paraffin sectioning.

Product series

Figure 1: Schematic Diagram of the Operation Process of the Organoid Embedding Gel

1. Organoid Collection
For organoids cultured in matrix gel, steps of degelling and fixation are required to obtain intact organoids (or degelling after fixation). Suspended organoids cultured without matrix gel can be directly collected and treated with tissue fixative. After centrifugation, remove the supernatant as much as possible.

2. Resuspending Organoids in SE - Gel
Place the organoids with the supernatant removed on ice. Add an appropriate volume of SE - Gel according to the number of organoids and the size of the tissue after embedding. The recommended dosage is 20 - 50 μL per gel droplet, and the recommended number of organoids is 3 - 5 organoids per μL.

3. Embedding and Solidification
Mark the sample names on the carriers in advance. Transfer the organoid/SE - Gel mixture to the carrier (Figure 2). After solidifying in a 37°C cell incubator for 5 minutes, flip the carrier (Figure 3) so that the mixture forms a suspended droplet under the carrier. Be gentle to prevent the embedding gel from falling off. Invert and place it in a 37°C cell incubator for further solidification, and continue incubating for 25 - 40 minutes. The time depends on the volume of SE - Gel. The larger the volume, the longer the time. During this period, it can be taken out to observe the solidification effect.

Figure 2: Embedding: In the figure, 50 μl of SE - Gel is used for embedding. After dropping it onto the carrier, its diameter is approximately 6 mm.

Figure A shows Method 1: Before the experiment, cut a piece of sealing film about 1 cm * 1 cm and stick it onto the carrier (the lid of a 24 - well plate), and then drop the corresponding volume of the gel droplet.

Figure B shows Method 2: That is, directly drop the gel droplet onto the carrier (the lid of a 24 - well plate).

Figure 3: Hanging Drop Method: After solidifying at 37°C for 5 minutes, gently rotate and invert the carrier. The red and black arrows in the above figure correspond to A and B in Figure 2 respectively.

4. Staining
After the SE - Gel turns milky white (when the cell content is low, the color is relatively light or even transparent), gently shake it. If the gel is in a fixed state without trembling, it indicates that the solidification is successful. Place the carrier upright and add a small amount of dye to cover the surface of each gel droplet. Stain at room temperature for 10 - 30 seconds (Figure 4).

6. Transfer
Add an adequate amount of PBS solution to the washed samples. Gently nudge the tissues using forceps, a pipette tip, or a pipette so that they float in the liquid. Transfer the samples to a new storage tube using the provided Pasteur pipette for preservation (Figure 6).

Figure 7: Frozen Sectioning of Organoids/SE-Gel.

A. Embedding and freezing with OCT.

B. The tissue resulting from the mixture of organoids and SE-Gel can be gently lifted with forceps.

C. Human gastric cancer organoids. Immunofluorescent staining for CEA.

Figure 8: Paraffin Sectioning of Organoids/SE-Gel.

A. Actual image of paraffin block embedding.

B. HE staining of human lung organoids, with 3μm sections.

During the tissue dehydration steps for paraffin and frozen sectioning, the gel droplets will shrink and become thinner, but this will not affect the subsequent operations or the structure of the samples.